Shallot (Allium ascalonicum L.) is an annual plant that is widely used as a mixture of cooking spices.  Shallot plants can grow in is 0-400 m above sea level, air temperature between 25-32⁰C and rainfall of 1000-1500 mm per year. Therefore it is necessary to cultivate shallots through organic fertilization in the form of Liquid Organic Fertilizer (LOF) and followed by right time of LOF application.  This study aims to determine the interaction of concentration and application time of LOF, determine the optimum concentration of LOF, and determine the appropriate application time of LOF. This research was conducted during March 2021 – May 2021 in Kandang Limun Village, Bengkulu City. This study used a completely randomized design of 2 factors and 3 replications. The first factor was the concentration of LOF which consisted of 4 levels, namely: K₀: 0 ml l^- ; K₁ = 3 ml l-, K2 = 6 ml l^- and K₃ = 9 ml l^-.  The second factor is LOF application time which consists of 3 levels, namely: W₁ = 1 Week after Planting (WAP), W₂ = 2 WAP, W₃ = 3 WAP.  Afterward there were 12 treatment combinations and replicated 3 times thus consist of 36 experimental units. Each experimental unit consisted of 3 sets then there were 108 polybags. The data obtained were analyzed using Analysis of Variance at the 5% level of the F test.  The results showed that there was no interaction between concentration and time of application of LOF on the growth and yield of shallots. There was no significant effect on the concentration of LOF for the growth and yield of shallots. There was no significant effect on the timing of application for the growth and yield of shallots